Abstract
We present a stereo-multispectral microscope equipped with an additional illumination unit allowing further narrow-band illumination in the spectral range of 400n.m up to 800nm. The combination of the normal microscope illumina-tion with the multispectral light unit allows different illumi-nation modalities to be realized, which enables intraoperative spectral tissue analysis with direct visualization. Two illumi-nation methods were tested in two cholesteatoma surgeries. In addition, two cholesteatom samples were illuminated and analyzed ex vivo. Cholesteatoma showed :fluorescent charac-teristics in our ex vivo analysis. This behavior could be used intraoperatively using a combination of white light and strong near-UV to blue illumination to highlight cholesteatoma tis-sue in the microscopic image. Thus, the visual differentiabil-ity of different tissue types can be improved and the clinical decision-making process can be accelerated.